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KMID : 0390320120220020067
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Chungbuk Medical Journal
2012 Volume.22 No. 2 p.67 ~ p.77
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Expression of VEGF-C, D and Lymphatic Density in T1 Gastric Cancer
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Yoon Jin
Yun Hyo-Yung
Song Young-Jin
Yonemura Yutaka
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Abstract
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Purpose: Abnormal expressions of VEGF-C and VEGF-D have been shown to play a role in the progression and the metastasis of tumors. We investigated the expressions of VEGF-C and VEGF-D to define the correlation with the clinicopathologic findings in T1 gastric cancer (EGC) and the lymphatic proliferation by measuring lymphatic vessel density (LVD)．
Materials and Methods: We examined formalin-fixed, paraffin-embedded archival tissues from 46 early gastric carcinoma (EGC) from Sizuoka Cancer Center in Japan. Tissue preparations were stained with rabbit polyclonal VEGF-C antibody, monoclonal anti-human VEGF-D antibody, mouse monoclonal D2-40 antibody. For more accurate information of lymph node metastasis (LNM) in EGC, we performed immunohistochemical staining of 1230 lymph nodes from resected early gastric carcinoma with monoclonal mouse anti-human cytokeratin 18 antibody to detect micrometastasis.
Results: VEGF-C and D expression rate were 63% and 34.5%. VEGF-C was closely related with the depth of invasion (p=0.003) and lymphatic invasion (ly) (p=0.002) but VEGF-D was related with the depth of invasion (p=0.046). When both VEGF-C and D expression were positive, strong correlation with ly was observed (p=0.000). When we compared lymphatic vessel density (LVD) among VEGF(+), VEGF(-) tumor margin and normal stomach tissue, LVD was significantly increased in VEGF(+) tumor margin(p=0.049).
Conclusion: Our results showed that expression of VEGF-C was related with peritumoral lymphatic proliferation and lymphatic invasion but not related with LNM. This finding suggests that VEGF-C expression has influence on the early stage of LNM in EGC.
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KEYWORD
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EGC, VEGF-C, VEGF-D, Cytokeratin-18, Lymphatic vessel density(LVD), Micrometastasis
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